Transcriptomic and metabolomic analysis of a non-<i>cyp51A</i> mutant azole-resistant <i>Aspergillus fumigatus</i> isolated from Ningxia, China.

ABSTRACT:

Background

Azole-resistant A. fumigatus (Aspergillus fumigatus) has been extensively documented both domestically and internationally, with mutations in the cyp51A gene identified as the predominant mechanism of resistance. However, the incidence of clinical non-cyp51A mutation-resistant A. fumigatus has gradually increased in recent years, and the resistance mechanisms remain unclear.

Methods

We isolated a non-cyp51A mutant azole-resistant strain of A. fumigatus, designated Af68, from Ningxia. The fungus was further characterized using MALDI-TOF mass spectrometry and DNA sequencing of the beta-tubulin gene and the calmodulin gene for molecular characterization. We analyzed the growth diameters and responses of the azol

SUBMITTER: Ma W 

PROVIDER: S-EPMC12516256 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature