Cdh2, a downstream target of Hes7, regulates somitogenesis by supporting FGF signalling.

ABSTRACT: In the segmentation clock, Hes7 expression oscillates synchronously in the presomitic mesoderm (PSM), regulating periodic somite formation. Despite intensive studies, the whole regulatory gene networks of the segmentation clock remain to be analysed. To identify the direct target genes of Hes7, we performed chromatin immunoprecipitation with sequencing analysis using an anti-Hes7 antibody and knocked out the identified genes from mouse embryonic stem cells carrying a Hes7 reporter. These cells were induced to differentiate into PSM-like tissue, and live imaging of Hes7 oscillations was conducted. Among the tested genes, Cdh2 knockout resulted in downregulation of fibroblast growth factor (FGF) signalling and premature cessation of Hes7 oscillations. Conversely, Cdh2 overexpression led to u

SUBMITTER: Jia X 

PROVIDER: S-EPMC12516320 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature