Profibrotic immune cells and fibro-adipogenic progenitors contribute to skeletal muscle extracellular matrix remodeling and fibrosis in cancer cachexia.

ABSTRACT: Cachexia, the loss of skeletal muscle mass and function with cancer, contributes to reduced life quality and worsened survival. Skeletal muscle fibrosis leads to disproportionate muscle weakness; however, the role of infiltrating immune cells and fibro-adipogenic progenitors (FAPs) in cancer-induced muscle fibrosis is not well understood. Using the colon-26 adenocarcinoma (C26) model of cancer cachexia, we sought to examine the changes to skeletal muscle immune cells and FAPs, which contribute to excessive extracellular matrix (ECM) collagen deposition. CD2F1 male mice (n = 35) were implanted with either 10⁶ C26 or CT-26 (weight stable; WS) cells. Skeletal muscle immune cell populations, satellite cells, and FAPs were examined using high-dimensional flow cytometry. Skelet

SUBMITTER: Cardaci TD 

PROVIDER: S-EPMC12516515 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature