Detection of single-cell enzyme activity by single-time-point stable isotope probing-mass spectrometry.

ABSTRACT: Elucidation of single-cell enzyme activity is essential for clarifying cellular heterogeneity and its causal relationship with cell fate. In the currently available methods for enzyme activity detection, the traditional multiple-time-point approaches using the Michaelis-Menten model are susceptible to prolonged measurement periods and thus compromise normal cellular functions, whereas the single-time-point approaches using Lineweaver-Burk plots are limited by sample inequality and difficulty of aliquoting a single-cell sample. Mass spectrometry (MS) has the attractive advantage of simultaneous detection of multiple molecular components. Among the MS-based assays, stable isotope probing (SIP)-MS has provided an idea of single-cell enzyme activity analysis using a single-time-point approach.

SUBMITTER: Wu X 

PROVIDER: S-EPMC12516761 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature