Whole-genome sequencing of Lassa virus from dry blood spots: a comparative evaluation.

ABSTRACT:

Background

Whole blood samples are often used to generate whole genome sequences, which provide valuable insights into the genetic make-up of viruses. However, the collection and management present significant challenges, particularly in remote and resource-limited communities, where maintaining a cold chain is often difficult and costly. The use of dry blood spots (DBS) is gradually increasing to overcome these logistical barriers with reduced biosafety constraints. We propose an alternative approach using native DBS Lassa virus (LASV)-positive samples as a substitute for whole blood.

Findings

Next-generation sequencing (NGS) was performed on RNA extracted from whole blood and DBS samples using Illumina technology. RNA concentration, cycle threshold (Ct) values and sequence

SUBMITTER: Bangura U 

PROVIDER: S-EPMC12516887 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature