Xiaoxianxiong Decoction Attenuates LPS-Induced Pneumonia in Mice by Inhibiting Inflammatory Response Through Activation of SIRT1.

ABSTRACT: Xiaoxianxiong decoction (XXXD) is a widely used clinical prescription effective in the treatment of pneumonia. The study investigates the efficacy and mechanism of XXXD in pneumonia. In the study, an in vivo pneumonia model was induced by lipopolysaccharide (LPS) in mice, and an in vitro pneumonia model was induced by LPS in A549 cells. The study assessed the potential therapeutic effect of XXXD by detecting inflammatory cells in bronchoalveolar lavage fluid (BALF), myeloperoxidase (MPO) activity, lung wet/dry weight ratio, and histology. ELISA kits were used to detect the release of inflammatory factors. Apoptosis was identified by TUNEL labeling and flow cytometry, and c-caspase3/caspase3 expression was analyzed by Western Blot. SIRT1 expression was detected by immunofluorescence labelin

SUBMITTER: Shi L 

PROVIDER: S-EPMC12516909 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature