Yimusake ameliorates corporal endothelial dysfunction by down-regulating the NLRP3 inflammasome-mediated NF-κB signaling pathway and inhibiting oxidative stress.

ABSTRACT:

Background

Yimusake, a traditional Uyghur medicine, can treat a variety of male diseases, but its effects and mechanisms on diabetes mellitus-induced erectile dysfunction (DMED) remain unclear.

Aim

This study was designed to investigate the key cytokines and mechanisms by which Yimusake ameliorates corporal endothelial dysfunction in DMED rats.

Methods

Firstly, normal rat penile corpus cavernosum endothelial cells (CCECs) were extracted and cultured in vitro, and the injury model was established after stimulation with 30 mM glucose for 24 hours. Subsequently, the cells were cultured in Yimusake drug-containing serum for 24 hours, and Sh-NLRP3 lentivirus was transfected for 8 hours. Cells were collected for the subsequent experiments. Next, the DM model was esta

SUBMITTER: Yang C 

PROVIDER: S-EPMC12516956 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature