Targeting RAGE with Nanobodies for Molecular Imaging of Cancers and Alzheimer's Disease.

ABSTRACT: The receptor for advanced glycation end products (RAGE) is a multifunctional cell surface receptor implicated in aging and the progression of chronic diseases, including cancer and Alzheimer's disease. Its interaction with advanced glycation end products (AGEs) promotes cellular stress and inflammation, underscoring the diagnostic and therapeutic relevance of targeting RAGE. In this study, we explored the potential of nanobodiessingle-domain antibodies known for high specificity, strong affinity, and deep tissue penetrationas molecular tools for RAGE-targeted applications. Using a phage display library, a panel of RAGE-specific nanobodies were isolated and characterized. Binding activity and affinity were evaluated through enzyme-linked immunosorbent assay (ELISA) and surface plasmon reson

SUBMITTER: Liang G 

PROVIDER: S-EPMC12517322 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature