Co-transformation of Aspergillus fumigatus: a simple and efficient strategy for gene editing without linking selectable markers.

ABSTRACT: Understanding the basis of fungal pathogenesis requires knowledge of pathogen biology that is built through studies of gene function and regulation. The critical first step in nearly all these studies is genetic transformation: the generation of targeted DNA sequence modifications through the introduction of exogenous DNA into the cell. For research focused on gene regulation, or where small precise mutations are desired, the maintenance of genomic context (i.e. surrounding DNA sequences) is important, as the disruption of flanking DNA elements can alter gene expression and confound results. This often makes the inclusion of selectable markers that are physically linked to the sequence of interest unsuitable and complicates the transformation process. Here, we present a co-transformation s

SUBMITTER: Walker BE 

PROVIDER: S-EPMC12517355 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature