Comparative Transcriptomic Profiling of Corneal Compartments Using Single-Cell and Single-Nucleus Sequencing.

ABSTRACT:

Purpose

Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) offer complementary advantages for corneal transcriptomics study. However, variations in cell-type distribution across platforms and the technical challenges in isolating corneal cells may lead to misinterpretation of biological conclusions. This study aimed to establish cell-type-specific recommendations for corneal transcriptomics.

Methods

We systematically evaluated scRNA-seq and snRNA-seq performance using matched human and mouse corneal datasets. The expression of Col8a1 and Kdr was measured by immunofluorescence.

Results

Compared to snRNA-seq, scRNA-seq enabled more accurate prediction of differentiation trajectory in corneal epithelial cells. Moreover, scRNA-seq capture

SUBMITTER: Zhao H 

PROVIDER: S-EPMC12517360 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature