Molecular Investigation of the p28 Gene of <i>Ehrlichia canis</i> in Infected Dogs From Ho Chi Minh City, Vietnam.

ABSTRACT: Ehrlichia canis (E. canis) is a tick-borne, obligate intracellular bacterium that causes canine monocytic ehrlichiosis (CME), a widely distributed infectious disease in dogs with variable clinical severity. While the 16S rRNA gene has been commonly used for detection, limited data are available on the genetic diversity of the immunogenic p28 outer membrane protein gene, particularly in Southeast Asia. Eighty-three canine blood samples were collected from various districts in Ho Chi Minh City, Vietnam, and screened using polymerase chain reaction (PCR) assays targeting the 16S rRNA and p28 genes. Ten representative p28-positive samples were selected for Sanger sequencing. Phylogenetic relationships were inferred using the maximum likelihood method. Genetic variability was asse

SUBMITTER: Kieu NTL 

PROVIDER: S-EPMC12517983 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature