Establishment and verification of reference intervals for Alzheimer's disease plasma biomarkers based on evaluation of pre-analytical procedures: a multicenter study.

ABSTRACT:

Introduction

To establish and verify reference intervals (RIs) for Alzheimer's disease (AD) plasma biomarkers amyloid beta (Aβ) 40, Aβ42, phosphorylated tau 181 (p-tau181), p-tau217, and total tau (t-tau) after defining optimal pre-analytical procedures.

Methods

Pre-analytical procedures were assessed, including storage of whole blood and plasma under different conditions and freeze-thaw times. RIs were established using 738 samples, with grouping by sex and age (≤ 44, 45 to 59, ≥60 years), and verified with 120 samples from four clinical centers.

Results

Samples stored at 2°C to 8°C for 22 to 28 h or -20°C to -80°C for 30 days were stable. Though sex/age differences in biomarker levels existed, they did not require partitioning. RIs of Aβ40, Aβ42, p-tau181, p-tau217,

SUBMITTER: Jiang W 

PROVIDER: S-EPMC12518040 | biostudies-literature | 2025 Oct-Dec

REPOSITORIES: biostudies-literature