Enzyme-Substrate Complex Formation and Electron Transfer in Nitrogenase-Like Dark-Operative Protochlorophyllide Oxidoreductase (DPOR).

ABSTRACT: Nitrogenase-like dark-operative protochlorophyllide oxidoreductase (DPOR) is a two-component metalloenzyme involved in (bacterio)chlorophyll biosynthesis. DPOR enables photosynthesis in photosynthetic bacteria by catalyzing the MgATP hydrolysis-dependent, stereoselective two-electron reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). This requires the repeated transient association of DPOR's two component proteins (BchL and BchNB), and involves a series of individual and unresolved sequence of events (including MgATP-hydrolysis, electron transfer, protein association/dissociation, substrate binding, etc.). DPOR shares structural and mechanistic similarities with nitrogenase, although the spectroscopic properties of Pchlide and Chlide permit the reaction to be followed i

SUBMITTER: Bedendi G 

PROVIDER: S-EPMC12518044 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature