Workflow for efficiently isolating microspore cultures of different rice genotypes by optimizing the callus induction medium.

ABSTRACT: As one of the most important staple foods in the world, rice plays a key role in global food security. Doubled haploid technology based on isolated microspore culture can shorten the time taken for rice breeding programs. However, this technology still faces many problems, such as genotypic dependency, low culture efficiency, and a shortage of skilled workers. In this study, 15 rice genotypes, comprising 12 japonica genotypes and 3 indica genotypes, were randomly selected for microspore culture research, and the effects of different callus induction media (CIMs) on callus induction were compared and the related plant regenerations were also shown. The results showed that maltose was the optimal carbon source and the CIM III was the best for callus induction by comparing the n

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12518337 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature