Image scanning microscopy based on multifocal metalens for sub-diffraction-limited imaging of brain organoids.

ABSTRACT: Image scanning microscopy (ISM) is a promising imaging technique that offers sub-diffraction-limited resolution and optical sectioning. Theoretically, ISM can improve the optical resolution by a factor of two through pixel reassignment and deconvolution. Multifocal array illumination and scanning have been widely adopted to implement ISM because of their simplicity. Conventionally, digital micromirror devices (DMDs)¹ and microlens arrays (MLAs)^2,3 have been used to generate dense and uniform multifocal arrays for ISM, which are critical for achieving fast imaging and high-quality ISM reconstruction. However, these approaches have limitations in terms of cost, numerical aperture (NA), pitch, and uniformity, making it challenging to create dense and high-quality multifo

SUBMITTER: Jo Y 

PROVIDER: S-EPMC12518604 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature