Detergent-resistant α-amylase derived from Anoxybacillus karvacharensis K1 and its production based on whey.

ABSTRACT: In the field of biotechnology, the utilization of agro-industrial waste for generating high-value products, such as microbial biomass and enzymes, holds significant importance. This study aimed to produce recombinant α-amylase from Anoxybacillus karvacharensis strain K1, utilizing whey as an useful growth medium. The purified hexahistidine-tagged α-amylase exhibited remarkable homogeneity, boasting a specific activity of 1069.2 U mg^-1. The enzyme displayed its peak activity at 55 °C and pH 6.5, retaining approximately 70% of its activity even after 3 h of incubation at 55 °C. Its molecular weight, as determined via SDS-PAGE, was approximately 69 kDa. The α-amylase demonstrated high activity against wheat starch (1648.8 ± 16.8 U mg^-1) while exhibiting comparatively low

SUBMITTER: Ghevondyan D 

PROVIDER: S-EPMC12518654 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature