CAR NK cell production from human cord blood NK progenitor cells is enhanced by stimulation of the IL-15 receptor.

ABSTRACT: Cord blood (CB)-derived chimeric antigen receptor (CAR) natural killer (NK) cells have demonstrated significant antitumor efficacy. We recently reported that CB-derived CAR NK cells predominantly originate from CD7⁺CD56^-CD34^-HLA-DR^-Lin^- NK cell precursors in CB. Here, we demonstrate that stimulating the interleukin (IL)-15 receptor on these NK precursors enhances the production of CAR NK cells from CB cells. In CB CD56^-CD34^-HLA-DR^-Lin^- cells, the IL-15 receptor was exclusively expressed on CD7⁺ NK cell precursors. Using K562 feeder cells that express not only 4-1BB ligand and membrane-bound (mb) IL-21 but also mbIL-15 significantly increased the production of mature NK cells from t

SUBMITTER: Kogue Y 

PROVIDER: S-EPMC12518728 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature