Vitamin A Deficiency Induces Congenital Vertebral Malformation via Retinoic Acid Signaling Mediated Sclerotome Dysplasia.

ABSTRACT:

Objective

Congenital vertebral malformations (CVMs) arise from abnormal sclerotome development. Our previous study has indicated that vitamin A deficiency (VAD) induced congenital spinal deformity in rats. However, the phenotype observed through x-ray and the mechanism were still unclear.

Methods

Rats in VAD group were fed with a diet without added vitamin A, while rats in control group were fed with a normal diet. After mating, embryos were collected, and neonatal rats were euthanized. Micro-computed tomography and x-ray were utilized to detect the vertebral malformation. We applied whole mount in situ hybridization to visualize the expression patterns of Pax1 and Raldh-2 in embryos. Laser capture microdissection combined RNA-seq of sclerotome was performed.

Results

SUBMITTER: Huang X 

PROVIDER: S-EPMC12518919 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature