RP-HPLC for simultaneous determination of water-soluble vitamins in leafy vegetables using ultrasonicated acid hydrolysis.

ABSTRACT: This study reports the development and application of a rapid and efficient reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous separation and identification of water-soluble vitamins (WSVs) such as B₁, B₂, B₃, B₆, and B₉ in green leafy vegetables (GLVs) from Chhattisgarh, India. A sample preparation method based on acid hydrolysis assisted by ultrasonication was optimized using 0.1 M HCl. Separation and detection of the vitamins were carried out using a C-18 column with a gradient elution of orthophosphoric acid (OPA) and methanol (MeOH), with UV detection at 270 nm. The method demonstrated excellent linearity (r ² > 0.993), low limits of detection (LODs) ranging from 0.06 to 0.15 μ

SUBMITTER: Tikeshwari 

PROVIDER: S-EPMC12519113 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature