NN15-017 promotes human pluripotent stem cell proliferation and generation.

ABSTRACT:

Introduction

The nutrient requirements for culturing human pluripotent stem cells (hPSCs) have been studied in relation to the development of chemically defined media. Most hPSC media contain FGF2, which is critical for hPSC growth and maintenance. Here, we investigated compounds that could substitute for FGF2 activity.

Methods

To identify compounds that can replace FGF2 and promote the proliferation of undifferentiated hPSCs, we screened our compound library using the hOCT4-EGFP reporter system and evaluated candidates using cell morphological analysis and OCT4 immunostaining as a marker of undifferentiated cells. We further evaluated the extent to which the selected compound can replace FGF2 through long-term culture (over 10 passages). We examined whether the compounds pr

SUBMITTER: Fujigaki-Yamazaki S 

PROVIDER: S-EPMC12519253 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature