Selected miRNAs in Urinary Extracellular Vesicles Show Promise for Early and Specific Diagnostics of Diabetic Kidney Disease.

ABSTRACT: Diabetic kidney disease (DKD) is a health burden that lacks specific and early diagnostic biomarkers. For their discovery, we sequenced urinary extracellular vesicle miRNAs in a type 1 diabetes cohort of males with and without DKD. The results were replicated by sequencing or qPCR in two independent cohorts and six published datasets, including type 1 and 2 diabetes, and both sexes. We also validated stable reference gene candidate miRNAs. Chronic kidney disease, hypertensive nephropathy, IgA nephropathy, polycystic kidney disease, kidney stones, prostate cancer and non-diabetic cohorts served as additional controls. MiRNAs changed due to urine collection type or centrifugation before storage were excluded. We analyzed differentially expressed miRNAs and their correlations with clinical me

SUBMITTER: Barreiro K 

PROVIDER: S-EPMC12519432 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature