Trehalose-Functionalized Magnetic Affinity Probe Provides Biochemical Evidence of Nanoparticle Internalization in Mycobacteria.

ABSTRACT: We developed a magnetic affinity probe (MAP), consisting of iron oxide magnetic nanoparticles (MNP) functionalized with a photoaffinity labeling agent perfluorophenyl azide (PFPA), to characterize the internalization of nanoparticles by Mycobacterium smegmatis. Two MAPs were synthesized: a trehalose-functionalized MAP, PFPA-MNP-Tre, and an ethanol-functionalized MAP, PFPA-MNP-OH. Following incubation of MAP with bacteria, the samples were irradiated to trigger covalent bond formation between PFPA and bacterial proteins. The captured proteins were isolated by cleaving the disulfide bond in the linkers and removing the magnetic nanoparticles by using a magnet. For PFPA-MNP-Tre incubated with M. smegmatis for 24 h, proteomic analysis revealed that the captured proteins are cytop

SUBMITTER: Perera HA 

PROVIDER: S-EPMC12519468 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature