Biocompatible Guanidine-Functionalized Compounds with Biofilm and Membrane Disruptive Activity Against MRSA.

ABSTRACT: Three guanidine-functionalized 3,4-dihydropyrimidin-2(1H)-imine compounds (5a, 5b, 5c) were synthesized from 3,5-diaryldiene-4-piperidone and evaluated for antibacterial and antibiofilm activity against Staphylococcus aureus, CA-MRSA and HA-MRSA. The compounds showed bacteriostatic effects (MICs: 2.34-4.68 μg/mL). In vitro antibiofilm potential was demonstrated by significant reductions in biomass and metabolic activity, and structural analyses via SEM and fluorescence microscopy. Ex vivo antibiofilm activity was confirmed in porcine skin model. RT-qPCR revealed downregulation of biofilm associated virulence genes, indicating a multifactorial mechanism. Confocal microscopy showed increased levels of extracellular DNA and proteins, suggesting disruption of

SUBMITTER: Fukuda de Castilho P 

PROVIDER: S-EPMC12519469 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature