Aokap9 gene knockout contributes to kojic acid synthesis in Aspergillus oryzae.

ABSTRACT:

Background

Kojic acid, a significant secondary metabolite primarily synthesized by Aspergillus oryzae, has extensive applications across various industries. It is imperative to identify effective gene targets that can enhance kojic acid production and to elucidate its biosynthetic regulation for yield optimization in A. oryzae.

Results

In this study, we identify the Aokap9 gene, which encodes an uncharacterized protein containing two transmembrane domains, as being closely associated with kojic acid synthesis. Disruption of Aokap9 resulted in increased kojic acid production, whereas mutations in either kojR or laeA within the ΔAokap9 background abolished synthesis, indicating that Aokap9 regulates kojic acid synthesis through kojR and laeA. Conversely, AozfA overexpression s

SUBMITTER: Qiu T 

PROVIDER: S-EPMC12519625 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature