Identification and characterization of a novel β-galactosidase active at low temperatures from the Antarctic fungus Tetracladium sp., expressed in Saccharomyces cerevisiae.

ABSTRACT:

Background

β-Galactosidases are widely used in the dairy industry to produce lactose-free milk and prebiotics such as galacto-oligosaccharides and lactulose. Since commercial β-galactosidases have optimal activity at 35 to 70 °C, β-galactosidases that are highly active at lower temperatures are desirable to reduce production costs and minimize microbial contamination in industrial processes. Potential sources of cold-active β-galactosidases are microorganisms living in cold environments such as Antarctica. The aim of this work was to identify genes encoding β-galactosidases from Antarctic fungi and express them in Saccharomyces cerevisiae for their characterization.

Results

By searching 16 ORFeomes from eight Antarctic fungi, an ORF encoding β-galactosidase was identified in

SUBMITTER: Gutierrez F 

PROVIDER: S-EPMC12519765 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature