Bioenergetics of cancer cells: insights into the Warburg effect and regulation of ATP synthase.

ABSTRACT: The study reported here offers new insights into the metabolic changes associated with the Warburg effect (i.e. aerobic glycolysis) in cancer cells and into the possible role of IF₁, the endogenous inhibitor of ATP synthase that preserves cellular energy when it works in reverse, hydrolyzing ATP. We investigated biochemical and main bioenergetic parameters in cell lines derived from three human tumors: osteosarcoma (143B), colon carcinoma (HCT116), and cervix carcinoma (HeLa). The combination analysis of cellular glucose consumption, lactate production, ATP-linked respiration rate, ATP level, cell culture medium acidification rate, and ROS level demonstrates that aerobic glycolysis is differently expressed by the three different types of tumor cells, although all cell types exhi

SUBMITTER: Del Dotto V 

PROVIDER: S-EPMC12519848 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature