Standardization of Measurable Residual Disease in Acute Myeloid Leukemia by Flow Cytometry: A Multicenter Study.

ABSTRACT:

Introduction

Measurable residual disease (MRD) is a strong predictor of the risk of relapse of acute myeloid leukemia (AML). Therefore, for use in clinical decision-making, methods for MRD assessment must achieve adequate accuracy, sensitivity, specificity, and reproducibility. Multiparametric flow cytometry (MFC) is the most widely used method for assessing AML-MRD, but its sensitivity varies considerably due to the differing approaches used across centers, in addition to the different experiences of flow cytometrists, especially during clonal evolution. This study aimed to standardize AML-MRD by MFC in a multicenter project involving 16 Brazilian laboratories.

Methods

In the first phase, specialists were trained in pre-analytical standard operating procedures (SOPs) and an

SUBMITTER: Ikoma-Colturato MRV 

PROVIDER: S-EPMC12519880 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature