Rad53 orchestrates divergent pathways governing genotoxic resistance, morphogenesis, and virulence in <i>Candida albicans</i>: Mechanistic decoupling through overexpression analysis.

ABSTRACT: The DNA damage response (DDR), an evolutionarily conserved surveillance mechanism enabling cells to detect genomic lesions and cell cycle arrest for repair, coordinates genomic stability through key effector kinases. In Candida albicans, our prior work characterized Rad53-mediated transcriptional reprogramming based on the RAD53 deletion strain, establishing its canonical DDR functions. However, Rad53 activation dynamically orchestrates cell cycle synchronization and repair coordination during genotoxic challenge. Investigating cellular modifications resulting from increased Rad53 functionality is essential for a deeper understanding of its regulatory response. To this end, we overexpressed Rad53 revealing its dual regulatory capacity in stress adaptation and developmental pl

SUBMITTER: Wang J 

PROVIDER: S-EPMC12520088 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature