Ozone ultrafine bubble water sterilizes Porphyromonas gingivalis and neutralizes its virulence factors.

ABSTRACT: Periodontitis is caused by Gram-negative bacteria. Porphyromonas gingivalis, a major Gram-negative periodontal pathogen, produces virulence factors such as gingipains, lipopolysaccharide (LPS), and lipoproteins, which contribute to tissue destruction. Ozone ultrafine bubble water (OUFBW) has been studied for its antimicrobial effects against various bacteria and toxin protein inactivation. This present study aimed to explore the effects of OUFBW on P. gingivalis and its virulence factors. OUFBW was generated and applied to P. gingivalis to assess bactericidal activity. OUFBW effects on morphological changes in P. gingivalis and its membrane vesicles (MVs) were analyzed using transmission electron microscopy. Gingipain activities in OUFBW-treated P. gingivalis culture supernatant was tested

SUBMITTER: Endo M 

PROVIDER: S-EPMC12520332 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature