Identification of cCMP- and cUMP-binding proteins using cCMP and cUMP coupled to agarose and biotin matrices.

ABSTRACT: There is increasing evidence for a role of cyclic CMP (cCMP) and cUMP as second messengers. In a recent study we showed that cCMP activates both purified cGMP-dependent protein kinase Iɑ (PKGIɑ) and cAMP-dependent protein kinase (PKA) isoenzymes with the regulatory subunits RIɑ and RIIɑ. PKARIɑ was identified as a cCMP-binding protein using cCMP coupled to agarose by immunoblotting and PKARIIɑ by MS analytic. In this study, we discovered PKARIɑ, PKARIIɑ and PKG as cCMP- and also cUMP-binding partners using cCMP- and cUMP-agarose. For the first time as well as cCMP and cUMP coupled to biotin matrices was used and from mouse lung tissue, A549 and HeLa cell lysates the identical proteins were also identified as cCMP and cUMP binding proteins. In proteomic approaches, three isoforms of PKG (PK

SUBMITTER: Wolter S 

PROVIDER: S-EPMC12520408 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature