Regulating Enzyme Activity via Microaggregates Mediated by Phase Separation.

ABSTRACT: Liquid-solid phase separation (LSPS) is a biomolecular segregation process forming solid-like aggregates that encapsulate and concentrate specific biomolecules, thus profoundly influencing biochemical reactions. Building on this phenomenon, we developed an enzyme-regulation strategy inducing LSPS of target enzymes to isolate them from substrates. As proof of concept, we created a novel D-Peptide mediated Microaggregate Degradation named DPMD to capture trypsin and chymotrypsin in the seroperitoneum during postoperative pancreatic fistula (POPF). DPMD is designed with cationic and hydrophobic residues and a β-sheet-forming motif for specific binding. The core principle is an entropy-driven peptide self-assembly that sequesters these proteases from the extracellular environment. Captured enz

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12520456 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature