An Elegant Method of One-Pot Ligation-Desulfurization for High-Yielding Chemical Protein Synthesis.

ABSTRACT: While native chemical ligation (NCL), combined with radical-mediated desulfurization, has enabled chemical syntheses of a vast array of proteins, the presence of aryl thiols as catalysts in the ligation mixture prevents performing both ligation and desulfurization reactions in one-pot, as aryl thiols are effective radical scavengers. Existing one-pot ligation and desulfurization approaches are not ideal as they rely on the use of inefficient alkyl thiols as NCL catalysts. Here an extremely efficient and impressively straightforward method is presented that utilizes bromoacetamide, in conjunction with N-acetyl cysteine, for selective quenching of arylthiol following NCL, enabling ligation and desulfurization in one-pot without any intermediate purification step. The reagent combination faci

SUBMITTER: Malik V 

PROVIDER: S-EPMC12520469 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature