Large-Scale Production of Transfusion-Ready Red Blood Cells From Induced Pluripotent Stem Cells.

ABSTRACT: There is a constant worldwide need for blood products, traditionally obtained from donations. In vitro red blood cell (RBC) production could supplement this demand and offer benefits such as thorough screening for improved safety, the possibility of genetic manipulation to restore genetic deficiencies, and therapeutic loading. Induced pluripotent stem cells (iPSCs) are a promising cell source for transfusable RBCs due to their immortality and independence from donors. However, current iPSC differentiation protocols-including both monolayer and embryoid body-based systems-have failed to produce sufficient erythroid cells (10^11-12 per unit) for therapeutic application, primarily due to developmental immaturity, inefficient enucleation (5-25%), and suboptimal, static culture condit

SUBMITTER: Varga E 

PROVIDER: S-EPMC12520481 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature