RelA Inhibits Embryonic Myogenesis by Coordinately Regulating a Novel Distal Enhancer of Myogenin.

ABSTRACT: Skeletal muscle is crucial for lifelong metabolic health, with its development initiating during embryogenesis and guiding later growth. Single-cell RNA sequencing of E13.5 mouse embryos suggests that maternal obesity impairs embryonic myogenesis, primarily during myotube formation, and is correlated with the downregulation of myogenin (Myog) expression. Spatial transcriptomic sequencing further confirms these findings. Conversely, maternal obesity induces the upregulation of RelA, a transcription factor and inflammatory signaling effector, in the myogenic cells of obese embryos. Additionally, single-cell ATAC sequencing suggests that a cis-regulatory region, located 4 kb proximal to the Myog promoter, exhibits coordinated expression with Myog and acts as an enhancer of Myog. In obese embr

SUBMITTER: Hossain MN 

PROVIDER: S-EPMC12520497 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature