Homeostasis of DNA Hemi-Methylation in Arabidopsis through Methylation Maintenance, DNA Replication, and Nucleosome Positioning Mechanisms.

ABSTRACT: The DNA methylome in eukaryotes is an equilibrium contributed by maintenance methylation, de novo methylation, demethylation, and DNA replication processes. The asymmetric hemi-methylation status is thought of as a transient intermediate state en route to full-methylation. However, the recent studies in mammalian cells suggest that hemi-methylated CG dyads in certain regions can be stably maintained and may serve as epigenetic marks. Compared to mammals, plants have more diversified methylomes often including non-CG dyads. In this work, hairpin BS-seq is performed to acquire dyad-resolution methylomes from Arabidopsis wildtype and methylation-deficient mutant lines. Analyses of methylomes across multiple lines reveal how different DNA methyltransferases influence the equilibrium of hemi- a

SUBMITTER: Chen H 

PROVIDER: S-EPMC12520501 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature