Divergent Delivery and Expression Kinetics of Lipid and Polymeric Nanoparticles across mRNA Modalities.

ABSTRACT: Messenger ribonucleic acid (mRNA)-based therapies, including conventional linear mRNA (linRNA), circular RNA (circRNA), and self-amplifying RNA (saRNA), are being developed not only for vaccination but also for protein replacement, gene editing, and regenerative medicine. However, these mRNA modalities differ in structure and function, and their interactions with current non-viral delivery systems influence their therapeutic efficacy. Here, the in vivo expression kinetics of linRNA, circRNA, and saRNA delivered via lipid nanoparticles (LNPs) or bioreducible poly(cystamine bisacrylamide-co-4-amino-1-butanol) (pABOL) polymer are systematically evaluated. At 0.5 µg, Venezuelan equine encephalitis virus (VEEV)-based saRNA resulted in higher total luciferase expression than 5 µg of linRNA or ci

SUBMITTER: Casmil IC 

PROVIDER: S-EPMC12520532 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature