Intestinal Epithelial-Derived USP13 Alleviates Colonic Inflammation by Suppressing GRP78-mediated Endoplasmic Reticulum Stress.

ABSTRACT: Inflammatory bowel disease (IBD) is a chronic condition in which endoplasmic reticulum (ER) stress and subsequent apoptosis of intestinal epithelial cells play significant roles. E3 ubiquitin ligases and deubiquitinases are crucial in IBD pathophysiology by modulating protein ubiquitination. This study investigated the regulatory role of a deubiquitinase ubiquitin-specific peptidase 13 (USP13) in a DSS-induced colitis mouse model and explored the underlying mechanism. Intestinal epithelial-specific Usp13 knockout (USP13^IEKO) mice were treated with DSS to induce colitis. Adeno-associated virus serotype 9 (AAV9) was constructed to overexpress UPS13 in colonic tissues. The results show that intestinal epithelial-specific USP13 knockout exacerbated DSS-induced colitis, linked to inc

SUBMITTER: Qian C 

PROVIDER: S-EPMC12520541 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature