Activation of ZBP1/RIPK3/MLKL-Dependent Necroptosis by Pseudorabies Virus Restricts Viral Infection in BV2 Microglia Cells.

ABSTRACT: Pseudorabies (PR) virus (PRV), also known as suid herpesvirus 1, is an alphaherpesvirus that causes huge economic losses in the global swine industry. Necroptosis, a caspase-independent programmed cell death, has been demonstrated to play an important role in regulating viral infections. Here, we showed that PRV infection triggered receptor-interacting protein (RIP) kinase (RIPK) 3/mixed lineage kinasedomain-like (MLKL)-dependent necroptosis in BV2 microglial cells. Mechanistically, PRV infection induced the formation of Z-DNA and upregulated the expression of Z-nucleic acid-binding protein 1 (ZBP1), leading to ZBP1-RIPK3 interaction that triggered the RIPK3/MLKL-dependent necroptosis pathway. Additionally, both RNA interference and protein overexpression experiments demonstrated th

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12520810 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature