Tailor-made 3D <i>in vitro</i> maturation of early antral follicles uncovers cumulus-cell transcriptomic driver signature to predict oocyte competence.

ABSTRACT:

Background

Assisted reproductive technologies (ART) are constrained by the limited pool of medium antral follicles. Early antral follicles (EAfs) are an emerging additional source coming either from cryopreserved ovaries or from in vitro folliculogenesis protocols. The EAfs have the advantage of being gonadotropin sensitive follicles enclosing fully grown oocytes that can be enrolled in ART using advanced IVM techniques. The present research has been designed using the validated 3-D follicle-enclosed IVM protocol to insight into EAfs ART competence by profiling the transcriptome of the surrounding cumulus cells (CCs) to uncover non-invasive predictors of oocyte developmental competence.

Results

Transcriptome analysis of 22-141 genes revealed 1-386 DEGs in CCs supporti

SUBMITTER: Rojo-Fleming C 

PROVIDER: S-EPMC12520894 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature