Mono- and Co-Culture Biofilms of Candida auris or Candida albicans With Staphylococcus aureus Regulate Cell Viability and Pro-Inflammatory Cytokine Expression Differently in Oral Cancer Cell Lines.

ABSTRACT:

Background

Candida species and Staphylococcus aureus are nosocomial pathogens associated with immunocompromised individuals, especially oral cancer patients. This study elucidates the effects of mono- and co-culture biofilms of Candida albicans , Candida auris and S. aureus on cell viability and pro-inflammatory cytokine expression in healthy epithelial cells (hTERT TIGKs) and oral cancer (ORL-48) cell lines.

Methods

Mono- and co-culture biofilms of C. albicans , C. auris and S. aureus , developed using static biofilm for 72 h, were collected and filter sterilized (biofilm filtrate). Test cell growth medium (TCGM) was prepared for hTERT TIGKs and ORL-48 by mixing 20% (v/v) biofilm filtrate with 80% serum-free media. The cells were seeded in 96-well plates, and TCGM was added

SUBMITTER: Wan Ahmad Kamil WN 

PROVIDER: S-EPMC12521062 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature