<i>Klebsiella pneumoniae</i> detection by a light-controlled one-pot RPA-CRISPR/Cas12a method.

ABSTRACT:

Background

Klebsiella pneumoniae (KP) is a significant pathogenic bacterium responsible for severe infections in hospitals. However, existing traditional detection techniques, such as culture and PCR, are relatively inefficient. Therefore, this study aims to establish a rapid and convenient method for detecting KP.

Methods

This study developed a single-tube detection method combining recombinant polymerase amplification (RPA) and light-controlled CRISPR/Cas12a. RPA primers were designed and screened for the rcsA gene of KP to effectively amplify the target. A light-controlled CRISPR/Cas12a system was created using crRNA modified with a photocleavable group (NPOM). The two systems were integrated into a single tube. Following RPA amplificati

SUBMITTER: Pan L 

PROVIDER: S-EPMC12521164 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature