Targeting the CCL28-STAT3-PLAC8 axis to suppress metastasis and remodel tumor microenvironment in colorectal cancer.

ABSTRACT:

Background

Chronic inflammation plays a critical role in the initiation and progression of colorectal cancer (CRC), establishing a close link between the inflammatory microenvironment with tumor invasion and metastasis. However, the regulatory mechanisms by which inflammation-related factors promote CRC progression remain largely unclear.

Methods

The biological significance of PLAC8 in colorectal cancer was investigated through clinical data analysis, mouse models of colitis-associated colorectal cancer, gene knockdown and overexpression, as well as cell migration and invasion assays. Additionally, bioinformatics analysis, activation and inhibition of PI3K/Akt and JAK/STAT3 signaling pathways, along with techniques including CUT&Tag, Western blotting, and qPCR, were employed

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12521221 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature