Advancing cell therapy manufacturing: an image-based solution for accurate confluency estimation.

ABSTRACT: Cell therapies represent a transformative approach for treating diseases resistant to conventional therapies, yet their development and manufacturing face significant hurdles within the biopharmaceutical sector. A critical parameter in the production of these therapies is cell confluency, which serves as both an indicator of biomass in adherent cultures and a determinant of product quality. However, existing methods for measuring confluency are often inadequate for the large-scale cultivation systems used in industry, and current software solutions lack comprehensive automation capabilities necessary for a manufacturing environment. This article introduces a novel image-based software application designed for accurate cell confluency estimation, integrated with a high-throughput microscopy

SUBMITTER: Mason J 

PROVIDER: S-EPMC12521239 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature