Analysis of polychlorinated alkanes in food by liquid chromatography-tandem mass spectrometry.

ABSTRACT: Polychlorinated alkanes (PCAs), the principal constituents of chlorinated-paraffin technical mixtures, are persistent, bioaccumulative pollutants that raise growing toxicological concern. Due to their complexity, PCA analysis in food remains analytically challenging, predominantly relying on high-resolution mass spectrometry applications. This study aimed to develop and validate a more accessible liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying PCA-C_10-17 in food commodities. Six reversed-phase columns were evaluated during the study, and phenyl-hexyl and biphenyl stationary phases provided superior separation of critical isobaric PCA homologues. Ammonium acetate (5 mM) was used as a mobile phase additive to promote the formation of acetate adduct

SUBMITTER: Perkons I 

PROVIDER: S-EPMC12521293 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature