Signaling pathways contributing to the transcriptional response to alkalinization in Komagataella phaffii: role of the transcription factors Crz1 and Rim101.

ABSTRACT: Promoters able to respond to moderate alkalinization in the industrially relevant yeast Komagataella phaffii have been proposed as a tool for the design of novel methanol-free platforms for heterologous protein expression. However, the molecular bases of such response are unknown. We identify here the transcription factors Crz1 and Rim101 in K. phaffii and use CRISPR/Cas9 techniques to create single and double mutant strains. While crz1 cells are strongly sensitive to Ca²⁺ ions, mildly sensitive to alkaline pH and hypertolerant to SDS, rim101 cells are markedly hypersensitive to Na⁺ and Li⁺ ions, to alkaline pH and to SDS. RNA-seq analysis revealed that mutation of RIM101 affects 55% of alkaline pH-responsive genes, whereas lack of Crz1 alters only 11% of t

SUBMITTER: Albacar M 

PROVIDER: S-EPMC12521412 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature