Host S100A6 inhibits ZIKV replication by degrading NS3 through lysosomal pathway.

ABSTRACT:

Introduction

Zika virus (ZIKV) is a mosquito-borne arbovirus. Maternal infection may cause severe complications such as neonatal microcephaly and neurological defects. To date, there is no clinically approved vaccine or specific drug against ZIKV infection. The host calcium-binding protein, S100A6, is a member of S100 protein family, regulates various cellular processes, and has been recognized as a host-dependent factor for Flavivirus infection.

Methods

S100A6 expression in host cells after ZIKV infection was detected by western blotting (WB). The effects of host S100A6 on ZIKV replication as indicated by the RNA and protein levels of nonstructural protein 3 (NS3) were detected by qRT-PCR, plaque assay, immunofluorescence assay (IFA), and WB respectively. The interac

SUBMITTER: Peng J 

PROVIDER: S-EPMC12521416 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature