A fully automated rotary microfluidic platform for high-throughput multiplex detection of respiratory tract pathogens.

ABSTRACT: Rapid, high-throughput, timely, multiplex diagnosis of respiratory-tract infections still relies on laboratory infrastructure, sequential assays, and trained personnel, thereby delaying targeted therapy and outbreak containment. In this study, a Fully Automated rotary microfluidic platform (FA-RMP) for high-throughput multiplex respiratory tract pathogens detection was presented. FA-RMP enables a true "sample-in, result-out" workflow through the integration of swab lysis, reagent partitioning, lyophilized reverse transcription loop-mediated isothermal amplification (RT-LAMP), and moving-probe fluorescence read-out, all encapsulated with a disposable microfluidic cartridge and paired with a 9 kg, four-channel benchtop reader. The FA-RMP enables parallel processing of 16 independent reaction

SUBMITTER: Zhang D 

PROVIDER: S-EPMC12521484 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature