Refined cuff technique minimizes surgical complexity in rat lung transplantation and improves outcomes.

ABSTRACT: Orthotopic lung transplantation in rats serves as a pivotal platform for exploring long-term allograft outcomes, yet technical complexity has limited its widespread application. Here, we present a refined surgical model that combines structured intraoperative modifications with tailored mechanical ventilation strategies to ensure procedural consistency and biologic fidelity. Key interventions-such as a custom leak-proof endotracheal tube, a temporally controlled reperfusion maneuver, and an optimized anastomotic sequence-collectively addressed critical challenges including airway obstruction, graft impaired perfusion, and venous fragility. The protocol demonstrated a high procedural success rate and reproducible long-term survival, with histopathological analysis confirming progressive chr

SUBMITTER: Sahin MF 

PROVIDER: S-EPMC12521552 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature