Neurodevelopmental Impact of Prenatal Stress: A Proteomic Analysis of Myelination Disruptions in the Avian Embryo.

ABSTRACT: Prenatal stress, mediated by elevated glucocorticoid (GC) levels, is a relevant modulator of fetal brain development and a known risk factor for neurodevelopmental disorders. Using the avian embryo as a vertebrate model, we injected corticosterone into the yolk on embryonic day 6 (E6) and assessed neurodevelopmental outcomes at day 14 (E14). Through deep proteomic profiling - quantifying over 6500 proteins - we uncovered a robust molecular signature of stress-induced disruption. Key myelin-associated proteins (myelin basic protein [MBP], PLP1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase [CNP]) were markedly downregulated, indicating impaired oligodendrocyte maturation. These proteomic shifts were corroborated by immunohistochemistry and qPCR. Pathway-level analysis pointed to altered MAPK

SUBMITTER: Gaertner B 

PROVIDER: S-EPMC12521880 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature