BRD4 Inhibitor Alleviates Recurrent Spontaneous Abortion via Regulating BRD4/STAT3/IL-17A Axis to Decrease the Th17 Cell Differentiation.

ABSTRACT:

Purpose

Recurrent spontaneous abortion (RSA) is an abnormal phenomenon that severely affects women's quality of life. Inhibiting Th17 cell differentiation can alleviate RSA. This research explored the mechanism by which BRD4 Inhibitor (BETi) suppressed the differentiation of Th17 cells to mitigate RSA.

Methods

PBMCs and Naive CD4⁺ T cells were induced to differentiate into Th17 and Treg cells. An abortion-prone pregnancy mouse model was constructed by intraperitoneal injection of lipopolysaccharide. The Th17/Treg ratio was determined by flow cytometry. The association between STAT3 and IL-17A promoter was investigated by ChIP and dual luciferase assays. Co-IP and yeast two-hybrid assays were used to determine BRD4 binding to STAT3. The markers of Th17/Treg cell di

SUBMITTER: Liu F 

PROVIDER: S-EPMC12522068 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature